S₃₃₀₇ can be used to control the vegetative growth of plants, promote the reproductive growth of plants and increase crop yield. In this experiment, different concentrations of S₃₃₀₇ were sprayed at seedling stage, branching stage, squaring stage and flowering period, and the yield was measured to screen out the best time and concentration to promote mung bean yield. In this experiment, water was as CK, S₃₃₀₇ at 60 mg/L, 90 mg/L and 120 mg/L were sprayed in 2016; S₃₃₀₇ at 90 mg/L 120 mg/L and 150 mg/L were sprayed in 2017. The experimental results of two years showed that the yield of mung bean was increased by spraying each concentration of S₃₃₀₇ on the leaf surface of mung bean in each period. The test results in 2016 showed that S₃₃₀₇ with a concentration of 120 mg/L sprayed on mung bean leaves at seedling stage had a more obvious stimulation effect. The 2017 trial confirmed the same results as in 2016. Therefore, the method of spraying S₃₃₀₇ on the leaf is suitable for increasing the yield of mung bean. The best spraying period was the seedling stage of mung bean, and the best spraying concentration of S₃₃₀₇ was 120 mg/L.