In order to identify the species of Trichoderma spp. in different Solanaceae vegetables soils, 97 strains of Trichoderma spp. were isolated from the soil of pepper, eggplant and tomato. Morphological and molecular identification methods were used to identify the strains. The results showed that four Trichoderma spp. species were identified, including Trichoderma harzianum, Trichoderma viride, Trichoderma koningiopsis and Trichoderma aureoviride.The distribution of strains in the three soils was different. T. harzianum and T. viride were distributed in the three vegetable soils. The number of T. harzianum strains was the most, with 46.39% of separation frequency. The results of this study provide a theoretical basis for the identification of the distribution of Trichoderma spp. in specific vegetables soils and the exploitation of biocontrol agent.