In order to investigate the expression of lipid metabolism genes FABP3、FABP4、CPT1 involved in porcine normal and cyst ovary, ovarian tissues, granulosa cells and oocytes were collected, and the expression of genes were detected by real-time fluorescence quantitative PCR. The results showed that the expressions of FABP3 and CPT1 in porcine cyst tissues were significantly higher than those in normal ovarian tissues, the expression of FABP3 in granulosa cells of cyst ovary was significantly higher than that of normal ovary, and the expression of CPT1 and FABP4 in porcine cyst oocyte were significantly higher than that in normal ovarian GV and MⅡ oocyte. This research would provide a basis for further study of the role of lipid metabolism genes in the development of porcine ovarian cysts.