In this study, 43 cucumber germplasm resources were used as experimental materials to evaluate the agronomic traits of leaf, fruit, flavor and yield. 12 high-yield germplasms, 6 germplasms with good flavor, 5 germplasms with high soluble sugar content and 6 germplasms with high vitamin C were obtained, which provide reference for the screening of breeding parents. H2 and H34 with excellent comprehensive performance could be selected as the key breeding or extension cucumber varieties in the future.