This study found a liguleless plant from the self-crossing offspring of backcross group of Huangzao4, and obtained the recessive liguleless inbred line Ji D69. Using the method of backcross conversion, the liguleless gene of Ji D69 was introduced into Ji V203 and Ji V152, the two parents of the hybrid Jidan 513, and obtained the liguleless inbred lines Ji V203/lg and Ji V152/lg. In 2017, the hybrids Jidan 513, Jidan 513/lg, Jidan 513/sm and Jidan513/sf were prepared by using Ji V203 and Ji V152 and their corresponding liguleless inbred lines. The characters were investigated and the yield of the hybrids was measured in 2018. We found that the plant type of the liguleless corn converged and the ear size was generally smaller; when the parents of one hybrid are both liguleless inbred lines, the yield of the hybrid was reduced because of the small ear size, while when one of the parents of the hybrid was a liguleless inbred line, the yield was increased due to the improvement of density tolerance. Therefore, the new liguleless breeding material has a great application value in dense resistance breeding.