The sorghum hybrid Jiza 319 and its female sterile line 515 A and its male restorer line 501 R were used as test materials to plant under the same environmental conditions. At the flowering stage, the RuBPCase activity and relative chlorophyll content in leave,root bleeding intensity, nitrate nitrogen intensity, ammonium nitrogen intensity, soluble protein intensity in root bleeding liquid were measured. The difference of these physiological indicators between Jiza 319 and its parents was analyzed. The results showed that RuBPCase activity in leave,root bleeding intensity, nitrate nitrogen intensity and ammonium nitrogen intensity and soluble protein intensity in root bleeding liquid of hybrid Jiza 319 were significantly higher than those of its female sterile line 515 A and its male restorer line 501 R, and there were 1.34 times, 1.24 times, 1.17 times, 1.16 times and 4.04 times higher than that of its high parents（P＜0.05）. Leaf RuBPCase activity,root bleeding intensity, nitrate nitrogen intensity and ammonium nitrogen intensity and soluble protein intensity in root bleeding liquid of hybrid Jiza 319 had significant heterosis of super high parents. Relative chlorophyll content in leaves of hybrid Jiza 319 had significant heterosis of super middle parents. The results showed that the photosynthetic metabolism and root absorption metabolism ability of the sorghum hybrid Jiza 319 were significantly higher than that of its parents, which laid a physiological foundation for its high yield.