The differences of nitrogen absorption and utilization efficiency of main millet varieties in Chifeng underlow nitrogen stress were studied by pot experiment and field cultivation experiment. The pot experiment showed thatthe root length of Chigu 6, Chigu 8 and Chigu 9 increased significantly compared with nitrogen treatment, and Chigu 5,Chigu 6 and Chigu 9 had strong tolerance to low nitrogen stress. The nitrogen use efficiency of Chigu 10 was the moststable. Chigu 5 and Chigu 6 had higher tolerance index to low nitrogen. Chigu 5 showed good tolerance to low nitrogenstress in agronomic traits, nitrogen accumulation and low nitrogen tolerance index. Field experiments showed thatChigu 6 and Chigu 8 had strong tolerance to low nitrogen stress. During the whole growth period of millet, low nitrogenstress had great influence on the height of jointing stage, and the accumulation of dry matter in heading stage, fillingstage and mature period had great influence on low nitrogen stress. Ear related agronomic traits were more susceptibleto low nitrogen stress than other agronomic traits. In panicle, the effect of ear length on other traits is relatively slowand other traits are more sensitive, and low nitrogen stress sensitive index can be used as an indicator of low nitrogentolerance in millet. The results of comprehensive pot experiment and field cultivation test showed that Chigu 5, Chigu 6 and Chigu 8 were resistant to low nitrogen, and were suitable for planting in relatively poor soil in Chifeng area.