In order to study the effects of doubled CO₂ concentration and warming treatment on stomatal characteristics of winter wheat, three climate chambers with precise control of CO₂ concentration and temperature were used to set control（CO₂ concentration was 400μmol/mol, temperature was 21℃/16℃）, doubled CO₂ concentration（CO₂ concentration was 800 μmol/mol, temperature was 21℃/16℃）, warming（CO₂ concentration was 400 μmol/mol, temperature was 26℃/21℃）. The results showed that the stomatal density of the adaxial and abaxial surfaces of winter wheat increased significantly with the increase of CO₂ concentration and temperature（P＜0.05）, and the increase of the abaxial surface was more prominent than that of the adaxial surface. The stomatal length, stomatal width, stomatal perimeter and stomatal area of the adaxial side were substantially decreased by 8.6%, 18.2%, 9.4% and 29.4% under the high CO₂ concentration（P＜0.05）, whereas the stomatal traits of the abaxial side were barely affected（P＞0.05）.The stomatal width, area and shape index of winter wheat increased significantly in the adaxial plane with the increase of temperature（P＜0.05）, but did not change significantly in the abaxial plane（P＞0.05）. Increasing temperature and CO₂ treatment reduced the spatial regular distribution scale of stomata on the abaxial surface of wheat leaves, but had little effect on the adaxial surface.