In this paper, potted experiment of medium and microelement fertilizers was carried out in the chernozem area by planting different maize varieties to provide basis for nutrient balance management and scientific fertilization in the chernozem area. The results showed that Mg, Mo, Cu and Zn had significant effects on plant height, dry matter accumulation and yield formation of the two cultivars, with an increase of more than 7.6%. For SD 19, adding Mg, S, Mo and Zn can significantly promote dry matter accumulation at seedling stage, with an increase of 9.3%-18.5%; Adding Zn, Si, Cu and B can significantly increase grain yield, with an increase of 9.4%-16.6%. For JD 209, the addition of S, Cu, Mg and Fe could significantly promote dry matter accumulation at seedling stage by 18.3%-31.8%. The addition of Mn, Mg, S and Mo could significantly increase seed yield by 20.4%-33.2%. Comprehensive analysis showed that the addition of most medium and trace elements significantly promoted the plant height, dry matter accumulation and yield formation of maize, but there were significant differences among varieties.