In order to identify the goals and provide references for the breeding selection of sprout varieties, twelve soybean genotypes selected by previous study were used for experimental materials. Under the processing conditions and technologies similar to industrial production, the characteristics of seed size and soybean sprouts were analyzed and compared systematically in the artificial climate incubator. The results showed that there were distinct differences in the 100-seed weight, hypocotyl length and diameter among various genotypes, but no relevance to the environmental factor and genotype-environment interaction effect. The percentages of soybean sprout with different quality levels can be used as selection criterion in soybean breeding for sprout. Damaged or cracked cotyledons and high percentage of low-quality sprouts were the primary cause of sprout quality reduction. The results of correlation analysis indicated that there were highly significant correlations among 100-seed weight, fresh weight of high-quality and average-quality sprouts, and hypocotyl diameter. There was a positive correlation between percentage of highquality sprouts and sprout yield. A very significant positive correlation existed between hypocotyl length and percentage of high-quality sprouts. The hypocotyl diameter had significant positive correlation with fresh weight of high-quality sprouts. This study can provide practical criteria for selecting progenies in breeding soybean varieties used for sprout production.