Frankia is is an actinomycete that can symbiotically form nodules with non-legumes and achieve biological nitrogen fixation. Due to its growth cycle, nutritional mode and physiological and biochemical characteristics, it is difficult to obtain pure culture strains in isolation and culture, which makes its research progress slow. Therefore,it is expected to explore new medium and new separation method. The study found that exploring new media and new separation methods is expected to be a new outlet. In this paper, the research status of symbiotic nitrogen fixation and isolation culture of Frankia is reviewed in detail, in order to provide reference for further research.