The widespread use of bio-fertilizers will have a positive impact on the improvement of cultivated land fertility and soil fertility environment. Based on the survey of 313 rice farmers in Jilin Province, the factors affecting the use of bio-fertilizer by rice farmers were analyzed by using binary logistic model. The results showed that age,farming years, household income and proportion of off-farm income had negative effects on rice farmers' bio-fertilizer use. Gender, education level, cultivated land management scale and agro-technical training had positive effects on rice farmers' use of bio-fertilizer. The number of household labor had no significant effect on rice farmers' biofertilizer use.