A single factor experiment was designed to screen out the optimum carbon and nitrogen sources based on investigating the quality of culture. It was found that the optimum carbon is corn flour and the optimum nitrogen source is yeast extract powder. Six C/N ratios（10∶1～15∶1） were designed based on controlling the carbon source content, it was shown that the optimum C/N ratio was 11∶1. The optimum liquid strain culture formula of Hericium coralloides was corn flour 15 g/L, glucose 5 g/L, yeast extract powder 5 g/L, potato 200 g/L, wheat bran 2 g/L, magnesium sulfate 1.5 g/L, dipotassium hydrogen phosphate 3 g/L and V_B10.01 g/L. The orthogonal test of L₂₅（4×5⁴） was designed to screen out the optimal culture conditions according to the optimum C/N ratios formula. The volume of liquid was 150 mL/250 mL, the inoculation volume was 3.5%, the inoculation age was 7 days, the rotational speed was 180 r/min, and the pH was 5.0. The liquid culture was prepared with the optimum C/N ratio formula and culture conditions, and the solid culture was used as the control to produce mushrooms in the intelligent mushroom house using integrated and innovative mushroom environment. The results showed that the fresh weight and dry weight of each bag increased by 26.22% and 35.35%. The results of this study laid the foundation for the factory production and provided scientific guidance for the expansion of H. coralloides.