In this experiment, the relationship between the dynamic change of chlorophyll and SPAD value, chlorophyll content and yield of potato was studied. SPAD value, chlorophyll a, chlorophyll b, total chlorophyll and yield index（tuber number per plant and tuber weight per plant） were determined by SPAD-502 tester and ethanol∶propanol mixture method. The mathematical model of correlation among indexes is established by means of correlation regression statistical analysis. The results showed that the change trend of chlorophyll content was similar, and the correlation between SPAD value and chlorophyll b was the highest, R²=0.877. The contents of chlorophyll b and total chlorophyll were significantly positively correlated with the number of tubers per plant. The results showed that the chlorophyll content and yield could be well predicted by SPAD value, which provided a theoretical basis for early selection of potato breeding.