It was studied that the effects of the in vitro and ex vitro rooting for the test-tube seedlings on the growth and development of blueberry. The results showed that there were no significant differences at net photosynthetic rate, transpiration rate, intercellular CO₂ concentration and stomatal conductance. There were also no significant difference at water use efficiency, chlorophyll content, soluble sugar content and soluble protein content. There were no significant differences at growth indicators including main stem diameter, number of new shoots, main stem length and leaf color index. The survival rate of transplanting reached 88% when the shoot buds with 5 to 6 pieces of expanded leaves were taken for rooting outside the bottle. The ex vitro rooting is a feasible method for the test-tube seedlings of blueberry.