In order to obtain the antibacterial and antioxidative activities of Flammulina flavonoids extracted by ul-trasonic assisted high pressure method and the optimal conditions for purification by D101 macroporous resin,theresin was used to purify the flavonoids extracted by ultrasonic assisted high pressure method in the paper.The purifi-cation conditions were optimized,and the ability of the purified flavonoids to inhibit Escherichia coli,Bacillus subtilis,Salmonella enteritidis and Staphylococcus aureus was detected.The ability of flavonoids to remove DPPH·,·O₂^-,·OHand 2,2-binitro-bis ABTS·was determined with Vc as a comparison.The results showed that the optimum condi-tions for the purification were as follows:the adsorption solution was prepared at a concentration of 1.2 mg/m L and ap H of 5,and the adsorption was carried out at a loading flow rate of 0.75 m L/min.After saturation,it was eluted with 70% ethanol 60 m L at 1 m L/min.The purity of the flavonoids obtained under these conditions was 2.71 times thatbefore purification.The Flammulina flavonoids extracted by ultrasonic assisted high pressure method have differentinhibitory effects on the four strains.The order of inhibition is:Escherichia coli＞Salmonella enteritidis＞Staphylococcus aureus＞Bacillus subtilis.The Flammulina flavonoids have certain scavenging ability to DPPH·,·O₂^-,·OH andABTS·,but weaker than Vc.It can be seen that the Flammulina flavonoids extracted by the method have antibacteri-al and antioxidative activities,and D101 macroporous adsorption resin has better effect on purifying Flammulina fla-vonoids.