In order to analyze the chromosome karyotype and chromosome production techniques of Longkuiza 8, the effects of low temperature pretreatment of sunflower root tip cells production was studied by using Longkuiza 8 as the experimental material. Photoshop and Microsoft Excel software were used to assist in the analysis of chromosome images. The results showed that the low temperature pretreatment of sunflower root tip cells could obtain more ideal chromosome production for karyotype analysis, and the chromosome number of somatic cells of Longkuiza 8 was 2 n=34, whose karyotype analysis formula was 2 n=2 x=34=24 m+6 sm+4 st.