The study aimed to investigate the ability of some bacteria strains to degrade the deoxynivalenol（DON） in order to facilitate the application of such strain in the biodegradation process of DON in grain. The screening approach to identify the best performing strain was done using DON as the only source of carbon added to inorganic salt medium for enrichment and domestication. The bacterial mixed strains were natural flora isolated and enriched from chicken and fish intestines, as well as from soil samples. The best performing bacterial strain was determined by measuring the DON degradation ability of individual isolates. The results showed that DON degradability by the strain JC-5 was significantly higher than that of other strains in inorganic salt medium. The degradation rates were 56.56 %, 91.12 % and 96.97 % in 12 h, 24 h and 36 h, respectively. Furthermore, when the JC-5 strain was added to the grain contaminated with DON, the toxin in the grain can also be degraded obviously. The degradation rates were 50.32 %, 84.84% and 90.66% in 12 h, 24 h and 36 h, respectively. Based on phenotypic, physiological and biochemical properties and genotypic determination, the bacterium was identified as Comamonas jiangduensis. Compared with the existing DON degradation bacteria, this bacterium demonstrated significantly high degradation efficiency, particularly when applied in grain. Therefore, it lays a foundation for further development of efficient degradation pathway of mycotoxin in grain.