In order to explore the effect of organic fertilizer substitution for chemical fertilizer（base fertilizer） on apple yield, the experiment of organic fertilizer substitution for chemical fertilizer in apple orchard was carried out.The treatments of 20%, 40%, 60% and 100% organic fertilizer substitution for chemical fertilizer（base fertilizer）were set up. The apple yield, input and output of different treatments and the partial factor productivity of fertilizer（PFP） under different treatments were analyzed. The results showed that the substitution of organic fertilizer for chemical fertilizer（base fertilizer） could increase apple yield and commercial fruit rate. The substitution of organic fertilizer for 40% chemical fertilizer（base fertilizer） and 60% chemical fertilizer（base fertilizer） could significantly increase apple yield. Comprehensive analysis shows that the average net income per 667 m² of organic fertilizer replacing 40% chemical fertilizer（base fertilizer） is the highest, and the amount of chemical fertilizer is reduced by 19.5%, which improves the PFP of chemical fertilizer and can achieve chemical weight Loss and increase efficiency.