In this study, Danjing17 were used for research on the effect of Biological Fertilizer on the nitrogen use efficiency and yield of rice, which indicates that agronomic nitrogen use efficiency decreased with the increase of nitrogen fertilizer at the same level of microbial fertilizer. Nitrogen use efficiency of rice was treated by W1N1＞ W1N2＞ W0N1＞ W0N2. Application of bio fertilizer treatment of cultivated rice nitrogen utilization rate and control significantly. The variation regularity of nitrogen partial factor productivity was identical with agronomic nitrogen utilization rate, that is to say, the nitrogen factor productivity of medium nitrogen treated rice was higher than that of high nitrogen treatment. In the different levels of nitrogen fertilizer increased bacterial manure could significantly improve the yield in medium nitrogen yield the most obvious effect.