The effects of spraying low（0.67 mL/L）, medium（1.33 mL/L） and high（2.00 mL/L） level selenium-enriched organic water-soluble fertilizer on selenium content, dry weight, selenium accumulation and yield, quality in wheat were investigated under field conditions. The results showed that:（1） The selenium content order was root＞stem and leaf＞spike, the selenium accumulation in stem and leaf was the highest.（2） Spraying selenium-enriched organic water-soluble fertilizer could increase the selenium content, selenium accumulation in each organ, but had different influences on dry weight; with the increase level of selenium-enriched organic water soluble fertilizer, the selenium content, selenium accumulation increased first and then decreased in root, decreased first and then increased in stem and leaf, showed a upward trend in spike.（3） Spraying selenium-enriched organic water-soluble fertilizer could increase the yield, number of spike, grains per spike, 1000-grain weight, but had different influences on crude protein, crude fiber, wet gluten, fat, Se content; spraying medium level Selenium-enriched organic watersoluble fertilizer had a better effect on yield, 1000-grain weight, Se content, the increasing ranges were 8.4%, 6.2%,240.0%, respectively.（4） Spraying medium level selenium-enriched organic water-soluble fertilizer had the best effects on yield and quality, met the standard of selenium-enriched wheat.