Abstract:In recent years, a leaf blight disease has been discovered in the main Camellia oleifera producing areas in Guizhou. In order to find the pathogen of leaf blight, we have repeatedly isolated, cultured and purified the Camellia oleifera, and finally identified the pathogen of leaf blight as Pestalotiopsis kenyana. The physiological characteristics of the pathogen of the leaf blight disease were studied, and the optimum growth temperature was 20~30 ℃, and the optimum pH value was 6~8. In order to further evaluate the effects of five indoor fungicides: Benzene · azoxystrobin,Oxime bacteria · tebutazol, Difenoconazole, Fluocyclic imidamine, and Fluomycetes · tebutazolol to mycelia growth inhibition rate, mycelia growth rate and spore germination. The results showed that the EC50 of Fluocyclic imidamine to mycelia growth was 0.2 μg/mL. The EC50 of virulence to spore germination was 0.95 μg/mL, which is significantly higher than the other four species.