In order to realize the reuse of blueberry wine distillers’ grains, a green recycling industry model of“fruit-wine-residue-tree-fruit”was established. In this study, blueberry wine distillers’ grains and cow manure were composted in a certain proportion. After in situ monitoring, sampling analysis and comparison with matrix A and matrix B, two kinds of commercial matrix cultivated by blueberry were analyzed to evaluate the suitability of blueberry fermentation distillers’ grains as substrate for blueberry cultivation after composting. The results show that:（1）After composting, the pH value of blueberry wine distillers’ grains increased from 3.28 to 5.86, which was close to the optimum value of blueberry growth. An EC value of 0.79 mS/cm is suitable for blueberries growth.（2）Ammonium nitrogen in compost was 120.44 mg/kg, 1.91 and 1.54 times of that in the commercial matrix A and matrix B, respectively. Available phosphorus was 40.96 mg/kg, 9.14 and 1.56 times of that of matrix A and matrix B, respectively. The available potassium was 968.17 mg/kg, 6.31 and 1.06 times that of matrix A and matrix B, respectively. The organic matter was 84.21%, and the organic carbon was 48.85%, 1.84 and 1.81 times of that of matrix A and matrix B, respectively.（3） The germination index of compost varieties was 78%, which was acceptable and basically non-toxic.（4） The contents of heavy metals（As, Hg, Pb, Cd and Cr） were all within the standard limits. The ammonium nitrogen, available phosphorus, available potassium, organic matter and organic carbon of the cultivated matrix formed by the composting of blueberry wine distillers’ grains and cow manure were better than those of matrix A and matrix B, which is more suitable for the growth of blueberry needs. Therefore, blueberry wine distillers’ grains are very suitable for the cultivation of blueberry substrate raw materials.