Red pears are favored by people and have a broad market value. The color of red pear mainly depends on the synthesis and accumulation of anthocyanin, and there are many factors affecting the biosynthesis of anthocyanin,among which light is one of the most important factors. In this study, different light treatments were carried out on the Pingguoli pear. Based on the transcriptomics analysis in the previous laboratory and the cloning analysis of the key genes regulating the synthesis of related synthetic pear anthocyanin, further select Myb4-like1, bHLH130-like,myb-related protein 306, bHLH96-like, Myb4-like2, MYB1 R1, bHLH113-like（pyrus_GLEAN_10011886）, WDR,bHLH147, bHLH113-like（pyrus_GLEAN_10005548）, bHLH57-like genetic variations in different PCR identification of light treatment, the results found Myb4-like1, bHLH130-like gene significantly increase trends, such as WDR differentially expressed genes and the same as the contents cyanine trend obviously. This may change the photoperiod under the regulation of light, thus changing the expression level of the gene, and thus affecting the synthesis and coloring of Pingguoli pear anthocyanin.