In order to understand the effect of the soil residue of Atrazine on the cucumber, this test simulates the residual concentration of atrazine in the soil（0～0.40 mg/kg） for seedling experiments. The results show,the concentration of atrazine soil in the range of（0～0.40 mg/kg） has no significant effect on the emergence rate of cucumber seeds.When the treatment concentration reached 0.10 mg/kg, the growth of cucumber seedlings began to be inhibited, and the underground part of the seedlings was more sensitive than the aerial part, and the inhibition rate was larger than that. When the concentration reached 0.30 mg/kg, the net photosynthetic rate decreased by 56% compared with the control, and the seedling mortality rate exceeded 50%, which seriously damaged the growth of seedlings. When the treatment concentration reached 0.40 mg/kg, the seedling mortality rate was close to 100%. With the increase of treatment concentration, the SOD activity, POD activity, malondialdehyde content and soluble sugar content of seedlings increased, chlorophyll content, net photosynthetic rate and root activity decreased.