To deep research the differences among hybrid lines from 1867 × Longshu 7 in DNA level and cytogeneti-cal level, and the test used 1867 and Longshu 7 as contrast, and analyzed DNA site difference of 34 lines by usingSSR, and the pollen fertility and chromosome configuration of 16 excellent lines selected, which could provide forbreeding qualitative materials of new potato varieties. The result showed that（1） Two SSR primers S118 and STI047 could identify 34 hybrid lines and their parents, which constructed SSR fingerprint.（2） The pollen fertility rate of hy-brid C2, C3, C4, C5, C9, C10, C11, C12, C14, C19, C20, C21, C23, C26, C29 and C30 was significantly different,ranging from 19.01% to 90.59%. In addition, line C2, C3 and C26 were significantly higher than the female parent "1867".（3） Chromosome configurations of the 16 hybrid lines was significantly different, ranging from 1.71% to14.80%. The frequencies of C2, C3, C5, C9, C11, C12, C14, C20, C21, C26 and C29 divalent configurations werehigher, and the proportion of C26 was the highest（14.80%）. In this study, SSR fingerprints of 34 potato lines wereconstructed, and the differences of chromosome configuration and pollen fertility of 16 excellent lines were deter-mined, providing abundant germplasm resources for potato cross breeding.