Leymus chinensis is an important native forage grass with strong adaptability to saline-alkali. In this study, the full-length gene of LcCDPK1 was cloned by EST and RACE, with an ORF of 1 599 bp, which encodes an unstable, weakly acidic hydrophilic protein containing 532 amino acids. And a phylogenetic tree is constructed with the LcCDPK1 sequence. This study would help to further understand the function of the CDPK protein family of Leymus chinensis and provide a basis for genetic improvement of plants’ saline-alkali tolerance with LcCDPK1.