In order to explore the suitable nitrogen application rate of different flue-cured tobacco varieties, five flue-cured tobacco varieties, such as K326, OX2028, NC2326 and Yunyan 87, Honghuadajinyuan, were used in four flue-cured tobacco producing areas of China. The effects of increasing or decreasing nitrogen application rate on the main chemical composition, development, yield and quality of flue-cured tobacco varieties were studied. The test results show that based on the local customary fertilization amount: The yield and output value per unit area of K326, Honghuadajinyuan and OX2028 were improved by increasing nitrogen application amount moderately. The increase or decrease of moderate nitrogen application had little effect on the chemical composition of flue-cured tobacco leaves of five tested varieties. Moderate increase of nitrogen application rate is beneficial to the improvement of aroma quality and smoking quality of K326 and Honghuadajinyuan, while the effect of increasing or decreasing nitrogen application rate on smoking quality of OX2028, NC2326 and Yunyan87 is not significant. Therefore, based on the local nitrogen application rate, increasing the nitrogen application rate of K326, Honghuadajinyuan moderately is beneficial to improve the economic benefits of flue-cured tobacco cultivation and improve the aroma quality of tobacco leaves.