The branching at the base of stem of purple flowering stalks is an essential yield trait of Brassica campestris L. The endogenous hormones have important effects on the branching process. In order to investigate the effects of three hormones（auxin, cytokinin and Strigolactone） related to branching on stem base of flowering stalks, the six generations of genetic groups were constructed to analyze the inheritance of some branch characters of stem base by using the enzyme linked immunosorbent（ELISA）. The results showed that the characters of stem base branch were dominant to the characters without branch, and the characters of stem base branch of F₂ segregation population were continuously distributed, which were quantitative characters and did not conform to the typical Mendelian inheritance law. There was no significant difference in auxin and cytokinin between the two parents, but there was a significant difference in the content of Strigolactone. Combined with the phenotypic difference analysis between the two parents, it was suggested that the Strigolactone might be the direct factor affecting the basal stem branching of purple flowering stalks. These results lay a foundation for genetic cloning and formation mechanism of stem basal branches of purple flowering stalks.