In this study, cottonseed shell was used as the main material and soybean meal residue was used instead of bran as the auxiliary material to study the application effect of soybean meal residue in seed production and cultivation of Pleurotus geesteranus. The results showed that: The mycelia of Pleurotus geesteranus could grow normally in the formula with soybean meal residue as auxiliary material. The mycelia growth rate of No. 2 formula（5% soybean meal residue） was not significantly different from that of the control group, which indicated that soybean meal residue could be used to produce the original and cultivated species of Pleurotus geesteranus. The fruit body of Pleurotus geesteranus could grow normally in the formula with soybean meal residue as auxiliary material. The fruit body yields of No. 2 and No. 3 formula（10% soybean meal residue） were not significantly different from that of the control group. The fruit body yield of No. 4 formula（15% soybean meal residue） was significantly lower than that of the control group. In conclusion, adding 5%-10% soybean meal residue in the formula is feasible and improves the cultivation efficiency of Pleurotus geesteranus.