The effects of different application methods and amounts of zinc（Zn） fertilizer on wheat grain yields and accumulation of Zn,iron（Fe） and selenium（Se） in grains were investigated to provide theoretical and technical basis for wheat grain micronutrient biofortification.In addition to the Se-rich fertilizer uniformly sprayed by the unmanned aerial vehicle（UAV）,the current split-plot field experiment included two soil Zn application rates（0 and 30 kg/ha;Z₀,Z₁） as the main plots and four foliar Zn application rates（0,4.5,9,13.5 kg/ha;F₀,F₁,F₂,F₃） as the split-plots.Wheat grain yields and grain Zn,Fe and Se concentrations were determined at maturity.The results indicated that:（1） Soil + foliar applications of Zn fertilizer increased wheat grain yields,spike length and number,and had no significant effects on ear number and thousand-kernel weight.Compared with Z₀F₀ and Z₁F₀,Z₁F₃ increased the wheat grain yield by 13.29%-19.37%.（2） Soil and UAV spraying applications of Zn fertilizer could increase grain Zn concentrations.The grain Zn concentrations in treatments of Z₀F₁,Z₁F₁,Z₁F₂ and Z₁F₃ were 43.00,43.20,54.23 and 41.07 mg/kg respectively,which had reach the target value of zinc biofortification 40-45 mg/kg and increased by 17.44%-55.08% when compared with the control.（3） The UAV Se-rich fertilizer foliar spraying in combination with Zn application could improve the nutritional quality of wheat grain Se and the grain Se concentrations ranged among 0.22-0.37 mg/kg,which meet the target value of Se biofortification 0.15 mg/kg.There was no significant effect on Fe concentrations in wheat grains.（4） Soil Zn application in combination with UAV spraying applications of Zn and Se fertilizer increased wheat revenue by 2,858.8-6,679.8 yuan/ha.Therefore,it is a more economical way to increase grain yields and Zn and Se concentrations in wheat grains in zinc-deficient soil by applying Zn fertilizer in soil and spraying Zn and Se fertilizer with UAV simultaneously,which has a better effect on improving nutritional quality of wheat micronutrient.