The potato has become the fourth major grain crop in China,with important strategic significance,and potato virus disease is one of the main diseases.In this study,the Coat Protein gene of potato virus S（PVS） was cloned,and the expression vector pET28b was ligated into E.coli to express and purify PVS CP.The polyclonal antibody against PVS CP was prepared by immunizing Japanese rabbits.The titer of the recombinant protein was 64,000times and the titer of the recognized virus was 32,000 times.Antibody specificity analysis indicated that the prepared polyclonal antibodies were only recognized by PVS and were not recognized by potato virus M,potato virus Y or potato leaf roll virus.The preparation of PVS polyclonal antibody provides technical support for the detection of potato virus and lays a foundation for the improvement of seed potato quality.