In this study,the biological control effect of Bacillus licheniformis BL-24 on tobacco anthracnose was studied.The tabular confrontation test of tobacco anthrax was carried out with BL-24 fermentation broth and ferment supernatant respectively.The results showed that the mycelium growth inhibition rate was 66.67% and 68.89% respectively.Through field experiment,cell experiments were carried out using fermentation broth,ferment supernatant,and high temperature sterilizing solution of B.licheniformis BL-24 in comparison with chemical pesticides.The results showed that the live bacteria of B.licheniformis BL-24 was more effective than the other two in the control of tobacco anthracnose,and the average control effect reached 56.28%.The difference between the control effect and the chemical agent carbendazim is not significant,indicating that B.licheniformis BL-24 is an excellent microbial biocontrol strain,which provides a new way for the prevention and control of tobacco anthracnose,and also provides a theoretical basis for the development of B.licheniformis biologics.