Soil salinization is the main abiotic stress factor that affects plant growth and distribution and limits grain yield. In this study, LcVHA gene obtained from Leymus chinensis was transferred into cultivar Bert through Agrobacterium-mediated soybean cotyledon node transformation system to improve the alkali tolerance of soybean.Together with PPT resistance selection, the transgenic plants were confirmed by PCR detection, southern blot, RTPCR analysis and Quick PAT/bar strip detection. A total of 4,000 explants were infected. 108 transgenic plants with LcVHA gene were obtained by molecular detection. The conversion rate was 2.7%. The copy number of foreign gene in transgenic plants varied from 1 to 5, and the single copy rate was 21%. The results for alkali tolerance test showed that the transgenic plants under alkali stress（300 mmol/L NaHCO₃） grew better than the non-transgenic control plants, and the alkali tolerance of transgenic soybean was increased. In this study, total 23 alkali resistant transgenic lines were obtained, which provided new materials for the soda alkali resistant transgenic soybean breeding.