In the experiment, six kinds of endophytic bacteria isolated from ginger fruit were antagonized by the sputum culture method, and the antagonistic effect on the mycelium of P. stipitis was studied. The results showed that the four ginger endophytic bacteria J₁, J₂, J₄ and J₅ have antagonistic effects on the storing pear black spot disease during the initial screening and rescreening. The antagonistic rate at the 5th day of rescreening were 6.20%,18.22%, 54.19% and 12.79%, respectively. The antagonistic effect of J₄ was the most obvious. At the 7th day, the antagonism changed, and the antagonism rates were 7.15%, 1.73%, 53.46% and 6.49%, respectively. However, the inhibitory effect of J₄ was still the most obvious. The colony diameter of P. elegans treated with J₄ endophyte had a significant difference with the control and other treatments respectively, and the inhibition of J₄ remains stable. The J₄ strain was identified as Bacillus subtilis by morphological observation, physiological and biochemical experiments and 16S rDNA sequence analysis. The experimental results provided a good basic theoretical study for the development and utilization of ginger endophytic bacteria J₄.