Different flue-cured tobacco varieties have some differences in nicotine accumulation capacity. In order to select high-quality flue-cured tobacco varieties suitable for production, Xiguanzhuang and Dongzhou village in Fei County, Shandong Province were selected as experimental sites in this study to compare the economic characteristics, conventional chemical components and sensory evaluation quality of 13 flue-cured tobacco varieties. The results showed that NC89, NC95, 2286, SC58 and Corker 176 were the five flue-cured tobacco varieties with high nicotine accumulation ability. Further experiments showed that NC89 and Coker 176 have higher nicotine content and better sensory smoking quality than CK（NC102）. Comprehensive analysis showed that under the premise of 14leaves remaining, the best quality tobacco varieties with high nicotine were NC89 and Coker 176 under same fertility treatment. The tobacco leaves produced by the above two varieties had higher nicotine content, better sensory smoking quality, higher proportion of upper and middle tobacco and higher output value.