In order to explore the genetic relationship among the germplasm resources of Kyoho grapevine series, the genetic diversity of 24 cultivars was analyzed by SRAP marker, and the cultivar identification diagram（CID） was constructed by using the manual cultivar identification diagram（MCID）. 12 primers were screened for PCR amplification of 24 cultivars. A total of 126 bands were generated, of which 99 bands were polymorphic bands（78.57%）.The average value of observed number of alleles（Na）, effective number of alleles（Ne）, Nei’s gene diversity（H） and Shannon’s information index（I） were 1.7857, 1.3581, 0.2176 and 0.3388, receptively. The genetic similarity coefficient among 24 cultivars ranged from 0.6190 to 0.9603, with a range of 0.3413. The above results indicate that the genetic differences among the tested cultivars were small. The dendrogram of 24 cultivars was constructed using UPGMA. They can be divided into 3 groups at genetic similarity coefficient of 0.72, of which there were 22 cultivars in Group I, while Group II was Olympia and Group III was Heifeng. CID of 24 cultivars was constructed using polymorphic bands by 6 primers, by using CID we can find the primers that distinguish any two cultivars, providing scientific basis for the identification of Kyoho grapevine series.