Iron is one of the essential trace elements in newborn animals. Iron nutrition has important biological significance for the growth and development of sow fetus and the metabolism of newborn pig iron. In this study, Glycine Iron was added to the iron deficiency model of pig kidney cells, and the expression of EPO, HIF-1 and VEGF was detected by fluorescence quantitative PCR to study the effect of Glycine Iron on iron metabolism gene expression in pig kidney cells. The results showed that DFO with appropriate concentration can effectively reduce the content of iron in pig kidney cells, and the model of iron deficiency in pig kidney cells was successfully constructed. Glycine Iron was added to iron deficient cells to effectively increase the iron content in cells. Glycine Iron was added to regulate the expression of iron metabolism genes EPO, HIF-1 and VEGF. Its signal transduction pathways need further study.