In this experiment, the strain were isolated and identified from clinical samples which were collected from the chicken farm in Nanyang City of Henan Province, and the resistance of Staphylococcus aureus from chickens was analyzed. The sensitivity of the isolated bacteria to 11 antimicrobial agents was determined by the microdilution broth method, the related resistance genes such as aminoglycoside modifying enzyme genes（aphA, acc（6'）-aph（2″）,ant（4’）-Ia）, erythromycin ribosomal methylase genes（ermA, ermB, ermC） and tetracycline-resistant genes（tetK,tetM） were detected by PCR. The results showed that 15 S. aureus strains were identified, the resistance rates to penicillin and erythromycin were 100%, and the tested strains were susceptible to both tigecycline and vancomycin with the susceptibility rate 100%. Also, 66.7% of the strains were multi-resistant, the resistances varied from 2 to 9kinds of the above drugs. The 80% of the isolates harbored ermC gene, 20% harbored tetK gene, and the rest was about 50%. This research shows that antimicrobial resistance of S. aureus is relatively serious. Therefore it is necessary that drug resistance and resistance gene should be enhancement of monitoring.