The photosynthetic physiological characteristics and fruit dynamic quality changes of the main blueberry cultivars of O’neal and Misty were measured and analyzed in Sui County of Hubei Province. The results showed that the chlorophyll a, chlorophyll b and total chlorophyll of Misty was higher than O’neal. The diurnal variation of photosynthetic rate of O’neal and Misty was bimodal curve, with obvious "photosynthetic noon break". O’neal and Misty had higher LSP and better utilization efficiency of strong light. The LCP of Misty was low, and showed strong adaptability to the light environment. The respiratory rate of Misty was lower than that of O’neal, and the organic matter accumulation was higher than O’neal. The horizontal and vertical diameters of the fruit growth stage of Misty were significantly larger than O’neal, and the soluble solid content was significantly larger than O’neal at late development stage.