In this study,CO₂,CH₄and N₂O standard gases mixtures and 12 m L vacuum flask was used as experiment materials,to study the influences of cap use frequencies （2,10,20,30,40,50,60 times）,storage temperatures （room temperature at 10-25°C,freezing at-18°C） and storage days （1-180 days） on the change of greenhouse gases（GHGs） concentration.The results showed that,compared with the gas samples stored for 1 day,the concentrations of CO₂,CH₄and N₂O decreased by 1.5%,0.5%and 2.0%when the store 60,120,90 days,respectively.The effects of storage days and storage temperature on the concentrations of CO₂,CH₄and N₂O were interactive.The storage days of the three gases under freezing conditions were 30,90,150 days,respectively,which were 10,30,130 days longer than that under room temperature.Therefore,field GHG samples that cannot be detected in time are recommended to be stored under freezing conditions for the accuracy of GHGs detection results.In addition,the cap use frequency had no effects on the concentration of CH₄and N₂O,but it did affect the concentration of CO₂.At freezing temperature,GHGs should be tested within 20-150 days,60-90 days and 30-60 days,when the cap was used twice,10-50 times and 60 times.