A method based on gas chromatographic method was established to detect iprodione in soil, vegetables and MSM culture medium. Iprodione in samples were extracted with acetonitrile by shocks and ultrasound, then cleaned up using Carbon/NH₂ SPE column, finally separated on a HP-5 column with temperature programming, and detected by electronic capture detector. Iprodione could be completely separated under this condition of GC-ECD,its retention time was 8.263 min, the correlation coefficient r value was 0.9934-0.9998, the recovery of the method were 87.6%-113.4%, the relative standard deviations were 3.2%-6.8%, and the detection limits of this method were 0.001 mg/kg. This method was rapid, accurate and it could be applied to detection of iprodione in multiple matrix.