In this study, different blueberry varieties mainly planted in Yunnan were used as the research object,and the antibacterial effects of their extracts on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were compared. The results showed that Emerald had the worst inhibitory effect on the three pathogenic bacteria,with the minimum inhibitory concentration MIC of 250 mg/mL and the minimum bactericidal concentration MBC of 1,000 mg/mL. Sharp blue and Biloxi showed the strongest inhibitory effect on Escherichia coli, with the MIC and MBC values of 15.625 mg/mL and 62.5 mg/mL, respectively. The MIC value and MBC value of Misti and Biloxi were 15.625 mg/mL and 62.5 mg/mL, respectively. The MIC value and MBC value of the two blueberry varieties against the two strains were the same, indicating that these blueberry varieties had similar inhibitory effects on the two strains. Legosi and Brilliant had the strongest bactericidal effect on Pseudomonas aeruginosa（MBC）, and their MBC value was 62.5 mg/mL. The MIC values of Sharp and Biloxi were the lowest（15.625 mg/mL）, while the MBC value of Brilliant was the lowest（62.5 mg/mL）. The results provided a theoretical basis for further study on the physiological activity of blueberry anthocyanins, and provided a theoretical basis for the application of blueberry polyphenols in food preservation.