Industrial hemp is one of the research hotspots in recent years, and its role is very extensive, but its research on the survival rate and the quality of cuttage is relatively rare. In this research, cutting technology was applied to industrial hemp. In order to explore the influence of different substrates and different micro-fertilizers on hemp cuttage, 12 substrate combinations and 20 micro-fertilizers were set up in this study. The results showed that in the substrate test, treatment 10（turfy soil:vermiculite:coconut husk = 2:4:4） had the best root development effect, followed by treatment 5（turfy soil:vermiculite:perlite:coconut husk = 4:2:2:2）, which had the highest fresh weight and dry weight. Treatment 1（turfy soil : vermiculite : perlite : coconut husk = 4:4:1:1） had the highest chlorophyll content and leaf nitrogen content, followed by treatment 5. In the micro-fertilizer experiment, treatment 18（Megafu） had the best root development effect. Treatment 7（Potassium Nitrate） had the highest fresh weight, and treatment 11（Hydrogen peroxide） had the highest dry weight. Treatment 13（Humic Acid） had the highest chlorophyll content and leaf nitrogen content. From a comprehensive analysis point of view, the combination（turfy soil:vermiculite:perlite:coconut husk = 4:2:2:2） has the most suitable substrate condition for hemp cuttage. The best type of micro-fertilizer is potassium nitrate solution.