Strains of endophytic bacteria with antibacterial activity were isolated from Aloe barbadensis by the streak plate method and dual-culture inhibition. Botryosphaeria dothidea, Fusarium verticillioides, Fusarium graminearum, Fusarium oxysporum, Verticillium dahliae were selected for the antifungal experiment. Inhibition rates for the of LH-01 strain to 5 fungi were all more than 74%. LH-01 strain was identified as Bacillus tequilensis through the physiological and biochemical characteristics, cellular and strain morphology, 16S rDNA molecular identification and phylogenetic analysis.