In order to study on the effects of different growth regulators on cuttage survival of Lycium ruthenicum branches. The annual branches were cut with indolebutyric acid（IBA） and naphthylacetic acid（NAA） concentrations of 0（CK）, 100, 150, 200, 250 mg/L, respectively, and the branches with different thickness were treated respectively. The soaking time of the thick branches（0.2-0.4 cm） was 24 h, and the soaking time of the twigs（0.1-0.2cm） was 12 h, 24 h, 36 h, respectively. The results showed that during the production process, the thick branches could be soaked with 24 h 150 mg/L IBA and NAA growth regulator solution, while the twigs could be soaked with 24 h 100 mg/L IBA and 36 h 100 mg/L NAA growth regulator solution, which had guiding significance for the cuttage propagation and seedling formation of Lycium ruthenicum Murr.