In order to reveal the chlorophyll content of different organs of wheat more accurately and efficiently, the flag leaf, floret and anther of wheat were used as materials in this study to extract chlorophyll from different tissues and organs of wheat by using 80% acetone grinding method（Arnon method）, acetone ethanol grinding method, and acetone ethanol extraction method respectively. The results showed that the absorption spectra of chlorophyll solutions extracted by different extraction methods were basically the same, but there were differences in extraction efficiency. Among them, the acetone ethanol grinding method was the best method for extracting chlorophyll from flag leaves and florets, while the acetone ethanol extraction method（extraction for 18 hours） was more efficient for extracting wheat anthers, which provided necessary technical support for accurate extraction and detection of chlorophyll from different tissues and organs of wheat.