A method for the analysis of nine sulfonamides antibiotics residues in biogas slurry was established by using ultra high performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）. The samples were extracted by Na₂EDTA. Liquid extract was purified by an Oasis HLB SPE column, Electrospray ionization（ESI+） and selective reaction monitoring（MRM） mode were used to detect and quantify by external standard method. The results showed that the Correlation Coefficient between the concentration range of 0.01-5.0 μg/mL, the linear curve of chromatographic peak area of 9 sulfonamide drugs were all greater than 0.9984, at the levels of 0.01, 0.05, 0.5, 2.0μg/L, the average recovery of the samples were 62.3%-93.1%, and the relative standard deviation was 3.64%-10.90%. The detection limit was 0.1-0.8 μg/L, and the limit of quantification（LOQ） was 0.3-2.6 μg/L. This method has good purification effect, high sensitivity and reproducibility. It is suitable for the determination of nine sulfonamides in biogas slurry.