In order to identify the biological characteristics of mycelium of anthracnose disease of sorghum, the growth rate method was used to study its growth conditions. The results showed that the agar medium of sorghum leaf decoction was most suitable for the mycelium growth of Colletotrichum sublineola, followed by starch agar medium and carrot agar medium. The suitable temperature was between 20 and 35 ℃, and the optimum temperature was 28 ℃,while the lethal temperature was 54 ℃, 10 min; The mycelium can grow in the range of pH 3-10, the appropriate pH-value of medium was 8-10 and the optimal pH was 9; The suitable carbon source for mycelium growth of C. sublineola was maltose, and the suitable nitrogen source were peptone and yeast powder. The biological characteristics of C. sublineola was studied in order to provide theoretical basis for effective control of the disease.