In this experiment, the mating behavior of Ostrinia furnacalis and biological characteristics related to its reproduction were studied. Results showed that the mating times of female O. furnacalis were relatively high, with an average of 4.25 times. The mating times of male moths were relatively low, with an average of 1.91 times. The mating times of O. furnacalis affected the longevity of its female and male moths. The life span of female moths shortened with the increase of mating times, and there was a significant negative correlation. The longevity of male moths prolonged with the increase of mating times, and there was a significant positive correlation. The age of O. furnacalis affected the mating rate of its female and male moths. With the increase of female moth age, mating rate first increased and then decreased, with quadratic curve correlation. When the age of male moth increased, the mating rate increased too and there was a positive correlation. In the application of controlling O. furnacalis, on the one hand, the mating age of female and male moths can be delayed to make them miss the optimal mating time so as to reduce the mating rate. On the other hand, through interference of the mating place and mating time between male and female moths, affecting the normal spawning of female moths, the population base can be reduced, thus achieving effective control.